Precision Denaturation| Odorless Reduction| Superior Resolution| Western Blot Optimized What Is LDS Sample Buffer (4X)? Atheris Bio™ LDS Sample Buffer (4X) is a chemically defined, high-performance buffer designed for denaturation, reduction, and loading of protein samples in SDS-PAGE and Western blot workflows. Optimized for controlled denaturation at 65°C, this formulation enables efficient protein unfolding with minimal aggregation, improving band clarity and reproducibility—especially for sensitive or complex protein samples. Its balanced Tris buffering system (pH 6.8 ± 0.05) combined with a low-odor reducing environment and stabilizing additives ensures reliable and consistent sample preparation across experiments. Quick Product Facts Attribute Specification Product Name LDS Sample Buffer (4X) Category Protein Sample Buffer Format 4X Concentrate Buffer System Optimized Tris-Based System Detergent LDS Reducing System Low-Odor, Stable (TCEP-based) Density Support Glycerol + Ficoll Enhanced Stability Stabilization System Betaine-Enhanced Protein Protection Tracking Dyes Coomassie G-250 (visual tracking) pH 6.8 ± 0.05 Storage 2–8°C Shelf Life 3 Months Format 1 mL Single-Use Intended Use Research Use Only (RUO) Key Features Optimized Denaturation at 65°CSupports uniform protein unfolding with reduced aggregation vs 95°C protocols Low-Odor Reducing System (TCEP-Based)Stable, user-friendly alternative to traditional reducing agents Enhanced Protein StabilityHelps maintain solubility during denaturation—ideal for sensitive proteins Improved Loading Performance (Ficoll + Glycerol)Ensures consistent sample density and sharp lane resolution Visual Tracking System (Coomassie-Based)Enables clear monitoring of sample loading and electrophoresis progress Chemically Defined & ReproducibleDesigned for consistent results across experiments and users Typical Applications SDS-PAGE sample preparation Western blot analysis Protein denaturation workflows Cell lysate preparation Membrane and cytosolic protein analysis Aggregation-prone or sensitive protein samples Compatibility ✔ Bis-Tris gels (optimized)✔ Tris-glycine SDS-PAGE gels✔ Precast gradient gels (TGX systems) ✖ Not suitable for native PAGE Atheris vs Basic LDS Sample Buffer Feature Atheris LDS Sample Buffer Basic LDS Sample Buffer Protein Denaturation Efficiency Optimized, minimal aggregation Standard, higher aggregation risk Thermal Stability Stable at 65°C Limited Reducing System Low-odor, stable (TCEP) Traditional, stronger odor Sample Loading Consistency Enhanced density (Ficoll + glycerol) Standard density Tracking Indicators Visual Coomassie tracking Single dye or none pH Control Dual-controlled, stable Possible drift Protein Compatibility Sensitive & complex proteins General proteins Shelf Life & Storage Stable (2–8°C) Variable Downstream Reliability High (sharp bands) Standard performance Storage & Handling Store at 2–8°C Mix gently before use Avoid contamination Use only if solution is clear Quality Control Each batch undergoes strict QC to ensure: pH verification (6.8 ± 0.05) Visual clarity and homogeneity Functional electrophoresis performance Formulation consistency Frequently Asked Questions Is LDS Sample Buffer compatible with standard SDS-PAGE workflows? Yes, it integrates seamlessly into conventional SDS-PAGE systems. What gel types work best? Optimized for Bis-Tris gels, and compatible with Tris-glycine and precast gradient gels. Is heating required? Yes, heating at 65°C is recommended for optimal protein denaturation. Does it replace traditional sample buffers? Yes, it is a high-performance, user-friendly alternative. Is this product for diagnostic use? No. This product is for Research Use Only (RUO). Defined Denaturation. Reliable Results.
