The methods described in the second edition onLegionellaare for the study of distinct features ofL. pneumophila. Chapters guide readers through ecology and physiology oflegionella, legionellagenetics, cellular microbiology oflegionella,biochemical assays to studylegionellaeffectors and enzymes, immunity and host response againstlegionella,metagenomics, proteomics, and host microbiomes oflegionella.Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Legionella: Methods and Protocols, Second Editionaims to be useful for scientists studyingLegionella, and for a broader research community interested in the ecology, physiology, pathogenesis, immunity, genetics and evolution of other bacterial pathogens.