Trypan Blue Stain 0.4% – Cell Viability & Cell Counting Reagent Trypan Blue Stain 0.4% is a vital dye used to assess cell viability by distinguishing viable from non-viable cells. Viable cells remain unstained (colorless), while nonviable cells take up the blue dye. This product is ideal for cell counting applications using LUNA brightfield counters.T13001 - Trypan Blue Stain 0.4% 2 x 1mL/200 tests Features & Benefits Standard 0.4% concentration, widely used in cell culture protocols Available in non-sterile and sterile-filtered versions for sensitive applications Compatible with LUNA™ II, III, FL, BX7 and FX7 automated cell counters Accurate, reliable viability assessment for research, diagnostics, or bioprocessing How To Use Mix equal volumes of cell suspension with Trypan Blue Stain (0.4%) Incubate at room temperature for 1-2 minutes Load into the cell counter / hemocytometer Count viable vs nonviable cells based on membrane integrity Note: Delay beyond 5 minutes can affect accuracy as viable cells may begin to uptake dye. Specifications Molecular formula: C₃₄H₂₈N₆O₁₄S₄ Molecular weight: 872.88 g/mol Appearance: Dark blue liquid Storage: Room temperature (protect from light) Ordering information Cat # Description Qty T13001 Trypan Blue Stain 0.4%, non-sterile 2 × 1.0 mL T13011 Trypan Blue Stain 0.4%, sterile-filtered 2 × 1.0 mL FAQ Why use 0.4% concentration?The 0.4% Trypan Blue is standard for viability tests. Lower concentration may under-stain non-viable cells; higher concentration may increase background. Can I use this with LUNA™ Brightfield counters?Yes. It is fully compatible with the LUNA™ II, III, FL, BX7 and FX7 counters. How long can the dye be stored?Refer to the MSDS / CoA for details. Once opened, avoid contamination; sterile-filtered version helps in such cases.
