.bio-chart { border: solid #3361ac 2px; text-align: center; } .bio-chart-title { background-color: #162f65; color: white; display: flex; padding: 3px 0px; } .bio-chart-content-1 { background-color: #ebf6ff; display: flex; align-items: center; padding: 3px 0px; } .bio-chart-content-2 { background-color: white; display: flex; align-items: center; padding: 3px 0px; } .chart-col-8 { width: 66.67%; } .chart-col-4 { width: 33.33%; } .chart-component { color: #3361ac; font-weight: bold; } .chart-parameter { color: #162f65; } .chart-col-6 { width: 50%; } .chart-col-3 { width: 25%; } Product Information Product description: M-MLV Reverse Transcriptase，RNase H Plus is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates. M-MLV Reverse Transcriptase, RNaseH Plus will also extend primers hybridized to single-stranded DNA. Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase.Source: Recombinant E.coliConcentration: 200U/μLUnit Definition: One unit is defined as the amount of enzyme required to catalyze the transfer of 1nmol of deoxynucleotides into acid-precipitable material in 10 minutes at 37℃.Storage Buffer: 50mM Tris-HCl (pH7.5, 25℃), 0.1mM EDTA, 100mM NaCl, 0.1%Triton X-100, 1mM DTT and 50% Glycerol.Companion Product: 5X RT Reaction Buffer, Cat#ON-067, 250mM Tris-HCl (pH8.3, 25℃), 375mM KCl, 15mM MgCl2, 50mM DTT. Key points of operation Before the first-strand Synthesis of DNA, heat the primer (about 0.5μg) and template (up to 2μg) to 70℃ for 5min to melt secondary structure within the template. Cool the tube on ice to prevent secondary structure from reforming. Add the following components to the annealed primer/template: Component Amount M-MLW reverse transcriptase, RNase H plus 50-200U 5X RT Reaction Buffer 5μL RNasin 20U dNTP (10mM each) 1.25μL Total 25μL Incubate for 60min at 37℃ for random primers or 42℃ for other primers. The extension temperature may be optimized between 37℃ and 42℃. Security Information Storage Conditions: -20℃ Quality Assurance: Free of endonuclease, exonuclease, Nickase and RNase activities. Physical Purity: ＞95% by SDS-PAGE. Reference 1. Yu Chen, Weiguo Xu, Qining Sun, Biotechnol Lett. 31, 1051–1057 (2009). 2. Monica J. Roth, Naoko Tanese, and Stephen P. Goff, The Journal Of Biological Chemistry. 260, 9326-9335(1985).
