Description Beef extract and peptone acts as source of carbon, nitrogen, minerals, vitamins and other essential growth nutrients. Mannitol is the fermentable carbohydrate. Phenol red is a pH indicator and change the color from red to yellow if mannitol is fermented and acid products are produced. Typical pathogenic staphylococci (coagulase-positive staphylococci) ferment mannitolandform yellow colonies with yellow zones around the colonies. Typical non-pathogenic staphylococci do not ferment mannitol andform red colonies. Sodium Chloride, in high concentration, inhibits most bacteria other than staphylococci. Cultural characteristics after 18-48 hours at 35-37°C. Components (for making 4.55L medium) Part I: Protease pepton 45.5g Sodium Chloride 341.25g D-Mannitol 45.5g Beef Extract 4.55g Phenol Red 0.115g Part II: Agar 68.25g Usage Must dissolve all contents of Part I in distilled water. Adjust pH=7.4-7.5, make final to 4.55L. Add Part II (Agar).Sterilize by autoclaving at 121°C for 15 minutes. Pour plates. Storage Store dehydrated powder, in a dry place, in tightly-sealed containers at 2-25°C. DG:No Storage:18 to 25℃ Sterile:No Documents Product Info
