Description Matrix Gla Protein (MGP) Human, E. coli Recombinant Protein is a single, non-glycosylated polypeptide chain containing 98 amino acids (20-96) and has a molecular mass of 11.8 kDa. The MGP is fused to a 20 amino acid His-Tag at N-terminus and is purified by proprietary chromatographic techniques. Summary Matrix Gla protein (MGP) is a member of a family of vitamin-K2 dependent, Gla-containing proteins. MGP has a high affinity binding to calcium ions, similar to other Gla-containing proteins. The protein acts as an inhibitor of vascular mineralization and plays a role in bone organization. MGP is vital for the regulation of calcification in the extracellular matrix, particularly in cartilage and arteries. Other Names Matrix Gla protein, Cell growth-inhibiting gene 36 protein, GIG36, MGLAP, NTI. Application Notes A study by Nishimoto S. K. & Nishimoto M. concluded that "cells attach and spread better on fibronectin and MGP coated surfaces than to fibronectin alone, even though MGP itself has no cell attachment activity." Fig. 1.: MGP augments cell spreading on fibronectin. The graph shows the calculated cell area/cell for fibronectin coated surfaces compared to fibronectin plus MGP coated surfaces. Source: Nishimoto, S. K., & Nishimoto, M. (2014). Matrix gla protein binds to fibronectin and enhances cell attachment and spreading on fibronectin. International journal of cell biology, 2014, 807013. https://doi.org/10.1155/2014/807013 Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MYESHESMES YELNPFINRR NANTFISPQQ RWRAKVQERI RERSKPVHEL NREACDDYRL CERYAMVYGY NAAYNRYF Type Recombinant Protein Source E. coli Purity Greater than 90%. Formulation Sterile-filtered colorless solution. The MGP solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol. Shipping Shipped on regular ice packs. Upon receipt, store the product at the temperature recommended below. Storage/Expiration Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. Usage This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive. RESOURCES Product Datasheet (PDS) Want to learn more about MGP? We have compiled the links below which contain information that you may find interesting. UniProt Knowledgebase: UniProtKB - P08493 (MGP_HUMAN): Matrix Gla protein PubChem Gene Summary: MGP - matrix Gla protein (human) Blog: Matrix Gla Protein Binds to Fibronectin and Enhances Cell Attachment and Spreading on Fibronectin Related Products Fibronectin Human, Purified Protein
