Specification Package 1250 ASSAYS Full name Micro BCA Protein Assay Kit Applications Used for protein quantitative testing Summary The BCA method is an ideal protein quantification method, which combines proteins with Cu2+in alkaline environments to reduce Cu2+to Cu1+. BCA combines with Cu1+to form a stable purple blue complex, which has a maximum light absorption value at 562 nm and is proportional to protein concentration. Based on this, protein concentration can be determined. This determination method has high sensitivity, simple operation, good stability of the reagent and its formed color complex, and is less affected by interfering substances such as detergents. This reagent kit is suitable for the determination of trace protein concentration. Features Suitable for quantification of low concentration protein samples; Quantitative range: spectrophotometry 0.5-20 μ G/ml; Enzyme labeling plate method 2-40 μg/ml; Compatible with ionic or non ionic decontamination reagents; The coefficient of variation for detecting different protein molecules is much smaller than the Coomassie brilliant blue method. Composition Reagent A, Reagent B, Reagent C, BSA standard solution (5 mg/ml) Assay BCA Assay Detection Method Colorimetric For Use With (Equipment) Spectrophotometer, Microplate Reader Component Reagent A, Reagent B, Reagent C, BSA standard solution (5 mg/ml) Storage Store separately according to label prompts
