Bcakground Leptin is secreted by white adipocytes into the circulation and plays a major role in the regulation of energy homeostasis. Circulating leptin binds to the leptin receptor in the brain, which activates downstream signaling pathways that inhibit feeding and promote energy expenditure. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation and wound healing. Mutations in this gene and its regulatory regions cause severe obesity and morbid obesity with hypogonadism in human patients. A mutation in this gene has also been linked to type 2 diabetes mellitus development. Specifications Synonyms LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein. Accession # P41160 Source Human embryonic kidney cell, HEK293-derived mouse Leptin protein Val22-Cys167 Predicted Moleucular weight 41.8 kDa Components and Storage Formulation Solution protein Dissolved in sterile PBS buffer This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening Storage and Stability Avoid repeated freeze-thaw cycles It is recommended that the protein be aliquoted for optimal storage 12 months from date of receipt, -20 to -70 as supplied Shipping Shipping with dry ice Quality Purity ≥95%, determined by SDS-PAGE Endotoxin Level ＜0.01 EU per 1 µg of the protein by the LAL method Activity Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human Leptin R. The EC50 for this effect is 0.075-0.45 ng/mL SDS-PAGE Bioactivity Documents Mouse LEPTIN
