Bcakground Mouse renin is an aspartic protease that is secreted by the kidneys. Renin is a part of the renin-angiotensin-aldosterone system involved in regulation of blood pressure, and electrolyte balance. This enzyme catalyzes the first step in the activation pathway of angiotensinogen by cleaving angiotensinogen to form angiotensin I, which is then converted to angiotensin II by angiotensin I converting enzyme. This cascade can result in aldosterone release, narrowing of blood vessels, and increase in blood pressure as angiotension II is a vasoconstrictive peptide. Specifications Synonyms angiotensin-forming enzyme; Angiotensinogenase; EC 3.4.23; REN; renin precursor, renal. Accession # P06281. Source Human embryonic kidney cell,HEK293-derived mouse Renin protein. Predicted Moleucular weight 41.8 kDa . Components and Storage Formulation Solution protein Dissolved in sterile PBS buffer . This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening. Storage and Stability Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 as supplied Shipping Shipping with dry ice . Quality Purity ≥95%, determined by SDS-PAGE . Endotoxin Stability ＜0.01 EU per 1 µg of the protein by the LAL method. . Shipping Measured by its ability to cleave the fluorogenic peptide substrate 5-FAM/QXL™ 520. The specific activity is >20 pmol/min/µg. SDS-PAGE Documents Mouse Renin
