PRODUCTION SPECIFICATION Product# 5101: Murine anti-gp130 SIV mac251 Monoclonal Antibody SPECIFICATIONS APPLICATIONS Antibody Class: IgG1 ELISA, Western ELISA Concentration: See vial Immunoprecipitation Mass/vial: 100µg Immunofluorescence Purity: >95% Immunohistochemistry Stabilizer: None Preservative: None Physical State: Frozen Liquid Storage: -75oC Stability: At least 12 months at -75oC. DESCRIPTION: High affinity murine anti-gp130 SIV mAb. Cross reacts with SIV mac239. SPECIFICITY: Binds to recombinant SIV gp130 and to SIV viral lysates in immuno blots assays and in Elisa and Western Elisa. APPLICATIONS AND INSTRUCTIONS FOR USE Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed at 1µg/ml of mAb depending on the amount of immobilized antigen in the assays. Western ELISA requires 1-5µg of mAb/ml. RELATED PRODUCTS PRODUCT#: 5101-P Peroxidase-Conjugated Murine anti-gp130 SIV Application: ELISA, Western ELISA, Histocytochemistry, RIPA. PRODUCT#: 5101-F Fluorescene Murine anti-gp130 SIV Application: FACS, Cell Staining of SIV infected cells.
