PRODUCT SPECIFICATION Product# 1106: Murine Anti-p66 (RT) HIV-1 IIIB Monoclonal Antibody SPECIFICATIONS APPLICATIONS Antibody Class: IgG1 ELISA, Western ELISA Concentration: See vial Immunoprecipitation Mass/vial: 100µg Immunocytochemistry Purity: >95% Diagnostics Stabilizer: None Preservative: None Physical State: Frozen Liquid Storage: -75oC Stability: At least 12 months at 75oC DESCRIPTION: High affinity murine anti-RT HIV-1 IIIB mAb. SPECIFICITY: Binds to native and recombinant RT in ELISA and Western ELISA. BIOLOGICAL ACTIVITY: Neutralizes HIV-1 IIIB RT as determined by HIV-1 RT assay in-vitro. APPLICATIONS AND INSTRUCTIONS FOR USE Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed at 10-100ng/ml of mAb depending on the amount of immobilized antigen in the assays. Western ELISA requires 1-5µg of mAb/ml. RELATED PRODUCTS PRODUCT#: 1106-P Peroxidase-Conjugated Murine anti-RT HIV-1 IIIB Application: ELISA, Western ELISA, Histocytochemistry, Histocytology. PRODUCT#: 1106-F Fluorescene Murine anti-RT HIV-1 IIIB Application: FACS, Cell Staining PRODUCT#: 1106-G Colloidal Gold-Conjugated Murine anti-RT HIV-1 IIIB Application: Dot Blots, EM. PRODUCT#: 1106-M Magnetic Murine anti-RT HIV-1 IIIB Application: Immunoprecipitation, Cell Panning.