PRODUCT SPECIFICATION Product# 1107: Murine Anti-vif HIV-1 IIIB Monoclonal Antibody SPECIFICATIONS APPLICATIONS Antibody Class: IgG1 ELISA, Western ELISA Concentration: See vial Immunoprecipivifion Mass/vial: 100µg Immunocytochemistry Purity: >95% Virus Neutralization Stabilizer: None Preservative: None Physical State: Frozen Liquid Storage: -75oC Stability: At least 12 months at -75oC DESCRIPTION: High affinity murine anti-vif HIV-1. SPECIFICITY: Binds to native and recombinant HIV-1 vif in ELISA and Western ELISA. BIOLOGICAL ACTIVITY: Neutralizes vif dependent HIV-1 replication in infected monocytic cells. APPLICATIONS AND INSTRUCTIONS FOR USE Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed at 10-100ng/ml of mAb depending on the amount of immobilized antigen in the assays. Western ELISA requires 1-5µg of mAb/ml. RELATED PRODUCTS PRODUCT#: 1107-P Peroxidase-Conjugated Murine anti-vif HIV-1 IIIB Application: ELISA, Western ELISA, Histocytochemistry. PRODUCT#: 1107-F Fluorescene Murine anti-vif HIV-1 IIIB Application: FACS, Cell Staining PRODUCT#: 1107-G Colloidal Gold-Conjugated Murine anti-vif HIV-1 IIIB Application: Dot Blots, EM. PRODUCT#: 1107-M Magnetic Murine anti-vif HIV-1 IIIB Application: Immunoprecipivifion.