eGFP is frequently utilized as a fluorescent tag for the observation and monitoring of protein expression and location within living cells. When eGFP-mRNA is transfected into cells, the enhanced green fluorescent protein (eGFP) is produced within the cellular environment. CleanRNA™ eGFP-mRNA (low dsRNA) is an mRNA that has been modified with a 5' cap1, a 3' poly(A) tail, and N1-Methyl-Pseudouridine Triphosphate (N1-Me-Pseudo UTP). The product is created through an in vitro transcription process using CleaScrip™ T7 RNA polymerase, which generates ultralow sRNA content in the mRNA solution, thus improving the in vivo performance of eGFP-mRNA. Specifications Catalog Number N120012S/N120012M Specifications 0.5 mg/1 mg mRNA length 995 nt Concentration 1 mg/mL Capping efficiency 100% dsRNA ＜0.1 ng/μg Storage buffer 1 mM sodium citrate Components Component Number Component Name Storage Temperature N120012S N120012M N120012 CleanRNA™ eGFP-mRNA (low dsRNA) -80°C 0.5 mg 1 mg Integrity tested by CE (capillary electrophoresis) Capping efficiency tested by MS Storage The products are shipped with dry ice and can be stored at -80℃ for two year. Instructions 1. Selection of transfection reagents. 2. Suitable for mRNA delivery, localization, and translation. 3. Applicable for analyses such as protein expression, cell activity, and in vivo imaging. Notes 1. Upon arrival, immediately store the samples at -80°C and avoid repeated freeze-thaw. 2. For the first use, be sure to wear disposable gloves, dissolve the samples on ice, and use RNase-free reagents and consumables to avoid contamination.
