NOTE: This product has been discontinued. We now offer PromedeusLab KL-6 (MUC1) ELISA, Human for KL-6 measurement. Description The Nanopia® KL-6 Assay, developed and manufactured in Japan by Sekisui Medical Co., Ltd., is a latex agglutination immunoturbidimetric assay for the quantitative measurement of sialylated carbohydrate antigen (KL-6) concentration in serum or plasma. Krebs von den Lungen 6 (KL-6) is a sialylated carbohydrate antigen that was detected by Kohno et al. in 1985. It is a high molecular weight glycoprotein that is expressed by type II alveolar epithelial cells and is a mucin which belongs to MUC-1 in cluster 9. KL-6 has been investigated as an important biomarker that is directly associated with the pathogenic process of interstitial lung disease (ILD), reflecting the extent of damage and regeneration of type II pneumocytes. It has been confirmed that the serum KL-6 level is significantly higher in subjects with interstitial pneumonia than in healthy volunteers or subjects with other respiratory diseases, and it has been shown by ROC analysis that serum KL-6 is a diagnostically useful indicator. Serum KL-6 levels may reflect connective tissue disease-associated interstitial lung disease severity. Type Immunoturbidimetric assay Other names Krebs von den Lungen 6, Sialylated Carbohydrate Antigen, Mucin-1, Mucin 1, MUC-1, MUC1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, CA 15-3, Carcinoma-associated mucin, Episialin, H23AG, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated epithelial membrane antigen, EMA, Tumor-associated mucin, CD227 Principle of Method Sialylated carbohydrate antigen KL-6 (KL-6) in samples agglutinates with mouse KL-6 monoclonal antibody coated latex through the antigen-antibody reaction. The change in absorbance caused by this agglutination is measured to determine the KL-6 level. Sample Types Serum, Plasma Sample Volume 2.5 µL Calibration Range 105.3 - 401.2 U/mL Dilution Linearity 50-5000 U/mL Within Run Precision 1.4 % Documents Reagent 1 & 2 product data sheets Calibrator product data sheet Control data sheet MSDS KL-6 Product Brochure KL-6 Components Available Cat. No. Description Size 466175 Nanopia KL-6 Reagent 1 2 x 24 mL 466199 Nanopia KL-6 Reagent 2 2 x 8 mL 536519 Nanopia KL-6 Calibrator Set 1 mL x 4 levels 516214 Nanopia KL-6 Control Set 2 levels, 3 x 1 mL each level KL6SET Nanopia KL-6 Complete Kit 466175+466199 +536519+516214 References to Summary Kohno N. Med J, Hiroshima Univ, 33, 971:1985. Kohno N. Respiration, 16, 391:1997. Kohno N., et al. Japan J Clin Exper Med, 75, 217:1998. Hirasawa et. al. Am J Resp Cell and Mol Biol, 17, 501: 1997. Lee, J.S., Lee, E.Y., Ha, YJ. et al. Serum KL-6 levels reflect the severity of interstitial lung disease associated with connective tissue disease. Arthritis Res Ther 21, 58 (2019). https://doi.org/10.1186/s13075-019-1835-9 Resources Want to learn more about KL-6? We have compiled the links below which contain information that you may find interesting: UniProt Knowledgebase: UniProtKB - P15941 (MUC1_HUMAN): Mucin-1 PubChem Gene Summary: MUC1 - mucin 1, cell surface associated (human) PubChem Protein Summary: Mucin-1 Scotto, Riccardo et al. “Serum KL-6 Could Represent a Reliable Indicator of Unfavourable Outcome in Patients with COVID-19 Pneumonia.” International journal of environmental research and public health vol. 18,4 2078. 20 Feb. 2021, doi:10.3390/ijerph18042078 Kondo, Tomohiro et al. “KL-6 concentration in pulmonary epithelial lining fluid is a useful prognostic indicator in patients with acute respiratory distress syndrome.” Respiratory research vol. 12,1 32. 22 Mar. 2011, doi:10.1186/1465-9921-12-32 Related Products PromedeusLab KL-6 (MUC1) ELISA, Human Yamasa SP-D Kit EIA II (96 wells) - Human Surfactant Protein D ELISA Yamasa Rat/Mouse SP-D Kit EIA II (96 wells) - Surfactant Protein D ELISA
