NEB MspI Description NEB MspI is a restriction endonuclease used for the sequence-specific cleavage of DNA during molecular cloning and genomic analysis. This enzyme is an isoschizomer of HpaII, specifically identifying and cutting the 5'-CCGG-3' recognition site. It is a critical tool for researchers investigating DNA methylation patterns, as its activity is unaffected by CpG methylation at the recognition sequence, allowing for the differentiation of methylated and unmethylated DNA states when used in conjunction with methylation-sensitive enzymes. Key Features Cleaves DNA at the 5'-CCGG-3' recognition sequence. Activity is insensitive to CpG methylation, enabling genomic mapping of methylated sites. Maintains high specificity and efficiency in standard molecular biology buffers. Suitable for double digest protocols with compatible restriction enzymes. Time-saver qualified for rapid digestion workflows. Applications Restriction site mapping and DNA fingerprinting. Epigenetic studies and DNA methylation analysis. Preparation of DNA fragments for molecular cloning. Characterisation of genomic DNA libraries. Specifications Recognition Site: 5'-CCGG-3' Isoschizomers: HpaII Methylation Sensitivity: Insensitive to CpG, Ccm, and 5-hmC methylation Optimal Reaction Temperature: 37 °C Heat Inactivation Temperature: 65 °C
