The Nikon CFI Super Fluor 20× Objective (NA 0.75) is a high-performance fluorite objective from Nikon’s Super Fluor series — delivering exceptional UV transmission below 340nm, high numerical aperture, and outstanding chromatic correction for demanding UV fluorescence applications including calcium imaging with fura-2 and indo-1, ratiometric dye imaging, and any workflow requiring high-quality fluorescence imaging from the UV through the visible spectrum. 🔬 Exceptional UV transmission below 340nm — the defining characteristic of the Super Fluor series, enabling high-efficiency excitation and emission collection for UV fluorophores including fura-2, indo-1, fluo-3, and DAPI 📊 NA 0.75 at 20× — high numerical aperture for excellent fluorescence light collection and resolution at intermediate magnification 🌟 Fluorite optical elements — superior chromatic and spherical aberration correction across the UV-visible spectrum for accurate multi-channel fluorescence imaging 📏 Working distance: 1.0mm — practical clearance for standard slides, coverslips, and imaging chambers ♾️ Infinity-corrected, M25 thread — compatible with Nikon CFI series microscopes including Eclipse Ti, Ni, E, and FN series 🇯🇵 Nikon quality, made in Japan — precision optical construction for consistent, high-efficiency light transmission and long service life ✅ New — catalog number MRF00201 🔧 Product Description The Nikon CFI Super Fluor 20× is part of Nikon’s Super Fluor objective series — a line specifically engineered for the highest UV transmission performance available in a standard infinity-corrected objective. The Super Fluor series achieves exceptional transmission below 340nm through the use of specially selected fluorite glass elements, making these objectives the definitive choice for UV fluorescence applications where standard fluorite or apochromat objectives have insufficient UV transmission. At 20× with NA 0.75, this objective provides an excellent balance of field of view and resolution for calcium imaging with ratiometric UV dyes (fura-2 excitation at 340/380nm, indo-1 excitation at 340nm), DAPI imaging, and any multi-channel fluorescence workflow that includes UV excitation wavelengths. The fluorite optical design also delivers outstanding chromatic correction across the full UV-visible spectrum, ensuring accurate co-registration between UV and visible fluorescence channels. Compatible with Nikon CFI series microscopes including Eclipse Ti, Ni, E, and FN series. Supplied new from stock, catalog number MRF00201. 🔎 Typical Applications Calcium imaging with fura-2 (340/380nm excitation) and indo-1 (340nm excitation) Ratiometric UV dye imaging requiring high UV transmission below 340nm DAPI and Hoechst nuclear staining with UV excitation Multi-channel fluorescence imaging combining UV and visible fluorophores Live-cell UV fluorescence imaging at intermediate magnification Any application where standard objectives have insufficient UV transmission 📊 Key Specifications Specification Details Catalog Number MRF00201 Magnification 20× Numerical Aperture (NA) 0.75 Working Distance 1.0mm Immersion Medium Dry (air) Optical Design CFI Super Fluor (fluorite, high UV transmission) UV Transmission Exceptional below 340nm Correction Infinity-corrected Thread / Mount M25 Compatible Microscopes Nikon CFI Eclipse Ti, Ni, E, FN series Country of Origin Japan Condition New SKU MRF00201 University and institutional purchase orders are welcome. Please contact us for invoicing and procurement documentation.
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