PRODUCT SPECIFICATIONS ITEM# 4005: Recombinant HCV 19kD NS4 Protein SPECIFICATIONS Concentration: see vial Mass/vial: see vial Volume/vial: see vial Diluent: 20mM Tris, pH8.0,10mM Beta Purity : >95% Stabilizer: None Preservative: None Storage: -75o C Stability: At least 24 months at -75o C DESCRIPTION: NS4 fragment - 130 aminoacids of HCV polyprotein expressed in the E. coli expression system, MW: approx. 20kD. APPLICATIONS: Western Analysis, In-Vitro HCV Diagnostics. PURIFICATION: HCV NS4 protein is purified by chromatography to >95% purity as determined by SDS-PAGE, reduced. SPECIFICITY: This protein binds to murine monoclonal antibodies specific for HCV NS4 protein. NS4 protein shows strong binding to HCV converted human serum polyclonal antibodies. BIOLOGICAL ACTIVITY: Not determined. APPLICATION AND INSTRUCTIONS FOR USE Recommended concentrations for use are approximate values. Dilute the antigen preparation at least 10 to 20-fold in PBS for solid phase antigen-antibody titration assays. A dose dependent response assay should be performed to determine the optimal concentration for use in specific application HCV core protein shows relatively strong biding specificity for HCV-specific human antibodies. ELISA and Western ELISA require protein in 10ng range depending on the nature and affinity of the secondary detection reagent. HCV converted human serum polyclonal antibodies yield titers of 1:10, 000 or greater at 100ng of immobilized protein under standard ELISA conditions.
