Synonym(s): Fungicidin, MycostatinDescription Nystatin is a fungistatic and fungicidal polyene antibiotic, which increases the permeability of the cell membrane of sensitive fungi by binding to sterols, chiefly ergosterol. Its main action is against Candida species. It is also effective against Aspergillus, Coccidioides immitis, Cryptococcus neoformans, Histoplasma capsulatum, Blastomyces dermatidis, and other yeasts and fungi. Nystatin has been used to enrich mutants by killing yeast cells. Nystatin has no antibacterial activity. The minimum inhibitory concentration for most sensitive fungi has been reported to range from 1.56 to 6.25 μg/ml. Nystatin is poorly absorbed from the gastrointestinal tract. It is not absorbed through the skin or mucous membranes when applied topically. Specifications Grade USP GRADE Formula C47H75NO17 MW 926.11 CAS 1400-61-9 Appearance Yellow or brown powder Identification I Positive Identification II Positive pH(3%,Water) @25C 6.5-8.0 Loss on Drying ≤5.0% Residue on Ignition ≤4.0% Abnormal toxicity Positive Assay ≥4500U/mg (dry) Storage -15~-20 ℃. Keep dry. Warm to room temperature before opening Application For the treatment of human macrophage cell line U937, to study the effect of nystatin on the production of IL-8 (interleukin 8) by silver nanoparticle-treated U937 cell line To determine its effect on intracellular accumulation of OxLDL (oxidized low density lipoprotein) mediated by SR-BI (scavenger receptor class B type I) in COS-7 cell line As a lipid raft modulator on mice macrophages to study the relationship between increased number of lipid rafts and the heightened response of TNF-α (tumor necrosis factor) Biochem/physiol Actions Mode of Action: Nystatin acts by binding to ergosterols in fungal cell membranes, increasing permeability of the membrane, and creating nystatin/ergosterol based ion channels. It also functions as a lipid raft-inhibiting reagent and as membrane associated cholesterol. Antimicrobial spectrum: Nystatin acts against fungi, yeasts and molds. Studies in severely burned patients have shown that treatment with nystatin powder is effective for the treatment of massive angioinvasive fungal infection, without side-effects on wound healing. Documents COA SDS Disclaimer: For laboratory research use only.
