Olympus UPLAPO 60× High Resolution TIRF Plan Apochromat Oil Immersion Objective (NA 1.50, Infinity) 🔬 60× magnification, NA 1.50 (oil) 🌟 Plan apochromat optical design — highest level of chromatic and flat-field correction 💡 TIRF-optimized for evanescent field excitation and super-resolution imaging 📏 Working distance: 0.11 mm 📷 Field number: 22 🔧 Standard RMS thread 🆕 New condition 🎓 University purchase orders accepted 🔧 Product Description The Olympus UPLAPO 60× HR TIRF Plan Apochromat objective (N5701600) is a high-resolution oil immersion objective engineered for the most demanding fluorescence microscopy applications. Part of Olympus’s high-NA TIRF series, this lens is among the first plan-corrected apochromat objectives to achieve NA 1.50 with standard oil immersion, delivering superior image flatness, high light throughput, and exceptional chromatic correction across the visible spectrum. The Plan Apochromat (UPLAPO) optical design represents the highest level of optical correction available, combining apochromat chromatic correction with flat-field (Plan) performance across the entire field of view. This dual correction is critical for quantitative fluorescence imaging, multi-color co-localization studies, and super-resolution techniques where both chromatic accuracy and field flatness are essential. With NA 1.50, this objective is optimized for TIRF and advanced super-resolution imaging techniques including TIRF-SIM, TIRF-RIM, and STORM. The ultra-high NA generates the critical angle required for total internal reflection, producing an evanescent field that penetrates only ~100–200 nm beyond the coverslip surface. This selectively excites fluorophores at the cell membrane while eliminating background from the bulk sample, providing maximum signal efficiency and minimal phototoxicity for live-cell, membrane, and single-molecule studies. 🔎 Typical Applications Total internal reflection fluorescence (TIRF) microscopy Super-resolution imaging (TIRF-SIM, TIRF-RIM, STORM, PALM) Single-molecule fluorescence imaging and tracking Membrane dynamics and surface interaction studies Live-cell fluorescence microscopy with minimal phototoxicity Receptor internalization and exocytosis studies 💡 TIRF vs Standard Fluorescence Objectives TIRF Objectives (e.g., this objective, NA 1.50) Ultra-high NA (1.45–1.70+) Excite only a thin surface layer (~100–200 nm) Extremely high contrast and low background Best for: Membrane imaging Single-molecule detection Live-cell surface dynamics Standard Fluorescence Objectives Lower NA (0.75–1.40) Illuminate entire sample depth More background fluorescence Better for: General fluorescence imaging Thick samples and tissue sections 📊 Key Specifications Specification Details Model Olympus UPLAPO 60× HR TIRF Product Number N5701600 Magnification 60× Numerical Aperture (NA) 1.50 Optical Correction Plan Apochromat Immersion Medium Oil TIRF Compatible Yes (optimized for TIRF-SIM, TIRF-RIM, STORM) Working Distance 0.11 mm Field Number 22 Mount RMS Optical System Infinity-corrected Condition New University and institutional purchase orders accepted. Expert technical support available. Contact us with any questions before ordering.
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