One-component TMB Substrate, 100mL Product Details Intended Use: TMB is a popular chromogenic substrate for HRP detection in ELISA and is available in several formats. The soluble One-component TMB Substrate are supplied as Ready-to-Use mono-component liquids that require no preparation before use. The product mainly contains 3,3’,5,5’-tetramethylbenzidine and contains no DMF or DMSO.One-component TMB Substrate yields a blue color (Amax= 370 nm and 652 nm) when reacted with peroxidase in micro-well applications. The product changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution. Method for use 1. Add 100 μL of the TMB Substrate Solution to each microplate well.2. Incubate for 5~30 min according to the reaction system.3. Stop reaction by adding 50 uL stop solution (1M HCl or 1M H2SO4).4. Measure the absorbance of each well at 450 nm. Storage 2-8℃, 12 monthsShading light. Product features Economical: Cost less compared with similar products.Convenience: mono-component, ready-to-use.High sensitivity: Save the dosage of antibody.Strong stability: Valid for 1 years at 2~8℃.Stable signal: The signal is continuous steady after adding stop solution.Safe: Non carcinogenic. Assay procedure 1. Add 100 μL of the TMB Substrate Solution to each microplate well.2. Incubate for 5~30 min according to the reaction system.3. Stop reaction by adding 50 uL stop solution (1M HCl or 1M H2SO4).4. Measure the absorbance of each well at 450 nm.
