Introduction Dulbecco's Phosphate-Buffered Saline (DPBS), 10X concentrated, without calcium chloride and magnesium chloride. The main purpose of buffered salt solutions is the maintenance of pH and osmolarity in cell culture applications as well as providing essential inorganic ions. These solutions are usually used for washing tissues and cells. Often they are combined with other agents in cell and tissue treatment. PAN-Biotech DPBS (10X) w/o: Ca and Mg is a 10x concentration, phosphate-buffered saline solution designed for use in cell culture research applications. It ensures reliable maintenance of pH and osmolality and provides essential inorganic ions required for safe and reproducible cell handling. Composition No calcium, no magnesium Component Concentration Potassium chloride: 2,000.00 mg/l Potassium dihydrogen phosphate: 2,000.00 mg/l Sodium chloride: 80,000.00 mg/l di-Sodium hydrogen phosphate anhydrous: 11,500.00 mg/l Specifications Cat. No. P04-53500 Quality: Research Use Only (RUO) Bicarbonate: Without Sodium Bicarbonate Ca/Mg: Without Ca and Mg Concentration: 10x concentrated Endotoxin Content: Endotoxin Content unspecified Liquid / Powder: Liquid Phenol Red: Without Phenol Red Product Category: Balanced Salt Solutions Product Type: DPBS Size: 500 ml bottles Sterile: Yes Storage Temperature: RT Manufacturer: PAN-Biotech GmbH Country of Origin: Germany Usage This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive. Documents Product Composition Material Safety Data Sheet (MSDS) Product References Havas, Fabien et al. “Hyaluronic Acid-like Skin Plumping and Radiance Benefits of a Porphyridium Sulfated Exopolysaccharide- and Natural PDRN-Rich Extract.” Marine drugs vol. 24,3 99. 1 Mar. 2026, doi:10.3390/md24030099 Dilek, Ozlem et al. “Small-Molecule Probe for Imaging Oxidative Stress-Induced Carbonylation in Live Cells.” Bio-protocol vol. 14,22 e5112. 20 Nov. 2024, doi:10.21769/BioProtoc.5112 Engelhardt, Jonas et al. “Lipopolysaccharide-Neutralizing Peptide Modulates P2X7 Receptor-Mediated Interleukin-1β Release.” ACS pharmacology & translational science vol. 8,1 136-145. 23 Dec. 2024, doi:10.1021/acsptsci.4c00496 Schneider, Anne T et al. “A decision point between transdifferentiation and programmed cell death priming controls KRAS-dependent pancreatic cancer development.” Nature communications vol. 16,1 1765. 19 Feb. 2025, doi:10.1038/s41467-025-56493-7 Skonieczna, Magdalena et al. “In Vitro Studies of Genistein Lipophilic Derivatives as Potential UV Radiation Protectors.” Pharmaceuticals (Basel, Switzerland) vol. 17,9 1166. 3 Sep. 2024, doi:10.3390/ph17091166 Bockstiegel, Judith et al. “P2X7 receptor activation leads to NLRP3-independent IL-1β release by human macrophages.” Cell communication and signaling : CCS vol. 21,1 335. 23 Nov. 2023, doi:10.1186/s12964-023-01356-1 Schlegel, Julian et al. “Biosensor Cell-Fit-HD4D for correlation of single-cell fate and microscale energy deposition in complex ion beams.” STAR protocols vol. 3,4 101798. 29 Oct. 2022, doi:10.1016/j.xpro.2022.101798 Frei, Lester et al. “Deep mutational learning for the selection of therapeutic antibodies resistant to the evolution of Omicron variants of SARS-CoV-2.” Nature biomedical engineering vol. 9,4 (2025): 552-565. doi:10.1038/s41551-025-01353-4 Bockstiegel, Judith et al. “The vasoconstrictor adenosine 5'-tetraphosphate is a danger signal that induces IL-1β.” Molecular medicine (Cambridge, Mass.) vol. 31,1 72. 21 Feb. 2025, doi:10.1186/s10020-025-01116-6 Dörschmann, Philipp et al. “Influence of a Very High-Molecular Weight Fucoidan from Laminaria hyperborea on Age-Related Macular Degeneration-Relevant Pathomechanisms in Ocular Cell Models.” Marine drugs vol. 23,3 101. 25 Feb. 2025, doi:10.3390/md23030101 Niklas, Martin et al. “Biosensor for deconvolution of individual cell fate in response to ion beam irradiation.” Cell reports methods vol. 2,2 100169. 17 Feb. 2022, doi:10.1016/j.crmeth.2022.100169 Karaca, Mawien et al. “Adverse outcome pathway-based analysis of liver steatosis in vitro using human liver cell lines.” STAR protocols vol. 4,3 (2023): 102500. doi:10.1016/j.xpro.2023.102500 Dörschmann, Philipp et al. “Specific Assay Protocols for Porcine Single-Eye Retinal Pigment Epithelium Concerning Oxidative Stress and Inflammation.” International journal of molecular sciences vol. 26,17 8434. 29 Aug. 2025, doi:10.3390/ijms26178434 Dörschmann, Philipp et al. “Porcine Single-Eye Retinal Pigment Epithelium Cell Culture for Barrier and Polarity Studies.” Cells vol. 14,13 1007. 1 Jul. 2025, doi:10.3390/cells14131007 Matziol, Troy et al. “Discovery of Novel Isoxazole-Based Small-Molecule Toll-Like Receptor 8 Antagonists.” Journal of medicinal chemistry vol. 68,4 (2025): 4888-4907. doi:10.1021/acs.jmedchem.4c03148 Related Products PAN-Biotech DPBS, without Ca and Mg, GMP Grade PAN-Biotech DPBS (1x), without Ca and Mg PAN-Biotech DPBS (1x), with Ca and Mg PAN-Biotech DPBS (10x), with Ca and Mg Balanced salt solutions Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech products.
