Description In many cases the isolation of cells is the first step for gene expression studies or in diagnostic procedures. Besides biological separation techniques physical separation methods are most commonly used. These methods use physical differences such as size and weight of the particles to be separated. For this purpose so-called separating solutions (also known as centrifugation media or density gradient media) are used. Pancoll separation media from PAN-Biotech contain a polysaccharide with a molecular weight of 400,000 daltons. This hydrophilic polymer allows production of aqueous solutions for cell separation with a density of up to 1.119 g/ml. PAN-Biotech offers a variety of ready-to-use products with a density of 1.063 g/ml up to 1.119 g/ml for a very wide range of cell separation applications. As the densities of blood fractions and the osmolality are different among species these parameters are optimized in Pancoll rat, Pancoll mouse and Pancoll animal. Specifications Pancoll Human: Cell Separation Medium, Density: 1.077 g/ml Density: 1.077 g/mL EDTA: without EDTA Endotoxin Content: Pancoll Human: Endotoxin Content unspecified * If you need low endotoxin Pancoll, please see Pancoll Pro (LPS 90 % live cells (trypan blue-exclusion) Other cells 3 ± 2 % Granulocytes 5 ± 2 % Erythrocytes < 0.5 % total number of platelets of the original blood sample Storage conditions & stability Store between 2° C to ambient temperature. Protect from light. When properly stored, density gradient separation media are stable for at least 36 months. The storage period starts with the manufacturing date. Resources Product Datasheet (PDS) Material Safety Data Sheet (MSDS) Pancoll Product References Related Products Pancoll Cell Separation Media Ilex Life Sciences LLC is an authorized distributor of PAN-Biotech products.
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