Description In molecular biology, cell culture, immunoassays, and protein analysis workflows, inconsistent buffer preparation can directly compromise sample stability, washing efficiency, and assay reproducibility. Manual formulation of standard buffer saline frequently introduces subtle variations in pH, salt concentrations, or trace nuclease contamination—errors that can ruin days of critical experimental benchwork. PBS Phosphate Buffered Saline (10X) pH 7.4 is an ultrapure, molecular biology-grade 10x phosphate buffered saline concentrate engineered to solve these laboratory challenges. Certified as RNase-free, DNase-free, and protease-free, this liquid PBS buffer eliminates preparation variance and saves critical time. Upon simple dilution to a 1X working concentration with deionized water, this premium phosphate buffered saline delivers a highly stable physiological environment containing precise concentrations of 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4 at pH 7.4, ensuring maximum experimental integrity for Western blotting, ELISA, and high-throughput cell assays. Specifications Parameter Specification Product Name PBS Phosphate Buffered Saline (10X) pH 7.4 Product Type Phosphate Buffered Saline (PBS) / Biological Buffer Concentration 10X Concentrate (Dilute to 1X for working solution) Volume 500 mL pH Value 7.4 1X Chemical Composition 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, 2 mM KH2PO4 Purity / Quality Grade Ultrapure Molecular Biology Grade; Certified RNase-free, DNase-free, and protease-free Nuclease Testing Rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity Shipping & Storage Ship at Room Temperature; Store at 15°C to 30°C Features Guaranteed Nuclease-Free: Certified free from detectable RNase, DNase, and protease activity, protecting sensitive DNA/RNA and proteins from degradation. Consistent formulation: Formulated via highly controlled processes to eliminate human errors caused by manual chemical weighing and pH adjustments. Time-Saving 10X Concentrate: Convenient concentrated phosphate buffered saline liquid format optimizes lab shelf space and permits rapid dilution preparation. Physiological Osmolarity Stability: Delivers defined NaCl, KCl, Na2HPO4, and KH2PO4 concentrations to mimic native in vivo conditions perfectly. Applications This molecular biology-grade 10x phosphate buffered saline serves as a reliable, high-purity PBS wash buffer, reagent diluent, or physiological salt solution across standard life science workflows: Cell Culture Washing: Used as a safe, isotonic PBS buffer for rinsing mammalian cells during routine handling and cell counting dissociation steps. Western Blotting: Ideal base buffer for membrane washing (e.g., preparing PBST), antibody dilution blocks, and general protein analysis. ELISA Assays: Provides optimal background reduction when utilized as a phosphate buffered saline solution for plate washing and specimen dilution. Molecular Biology Procedures: Perfect for genomic DNA/RNA extraction protocols, molecular diagnostics, and maintaining structural enzymatic stability. Immunoassay Preparation: Standardized buffer saline solution suitable for antibody-based immunofluorescence (IF) and immunohistochemistry (IHC) staining. Ordering Information Product Name Concentration pH Size / Volume PBS Phosphate Buffered Saline 10X 7.4 500 mL Frequently Asked Questions (FAQ) What is PBS Phosphate Buffered Saline (10X) used for? PBS Phosphate Buffered Saline (10X) concentrate is diluted to a 1X PBS buffer for everyday workflows such as cell culture washing, Western blotting, ELISA, immunoassays, sample rinsing, and molecular biology procedures requiring a strict nuclease-free environment. How do I prepare a 1X PBS working solution from this 10X concentrate? To make 1 Liter of 1X PBS working solution, combine 100 mL of the 10X PBS concentrate with 900 mL of sterile, deionized water. Mix thoroughly. The solution is pre-formulated to achieve a stable pH of 7.4 automatically—no additional pH adjustment is required. What is the shelf life of the diluted 1X buffer saline? While the sealed 10X concentrate remains highly stable at room temperature until expiration, once it is diluted to 1X working buffer saline, we highly recommend storing it at 2°C to 8°C and utilizing it within 1 to 2 weeks, or passing it through a sterile filter to completely eliminate risks of microbial growth. { "@context": "https://schema.org/", "@type": "Product", "name": "PBS Phosphate Buffered Saline (10X) pH 7.4", "image": [ "https://biofargo.com/cdn/shop/files/54c6fa12-7404-4be2-a0f9-20f243815281_1724913118344398573_tplv-a9rns2rl98-web-image_ee95cb14-94a3-4d92-9243-1249cf5b47bd.png?v=1732879187" ], "description": "Molecular biology-grade PBS Phosphate Buffered Saline (10X) pH 7.4 concentrate for dilution to 1X PBS. Certified RNase-free, DNase-free, and protease-free. 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