This vector contains red fluorescent protein - DsRed- driven by cytomegalovirus (CMV) promoter for high level expression in mammalian cells. The multiple cloning site (MCS) is located at C terminus in frame with DsRed. This enables the generation of N-terminal DsRed fusion proteins. Plasmid carries ampicillin resistance for bacterial selection and neomycin resistance for stable selection with G418 in eukaryotic cells. Total vector size 6102 bp. Research Use Only (RUO). Applications Generation of N-terminal DsRed fusion proteins Protein localization studies Live-cell fluorescence imaging Subcellular trafficking analysis Flow cytometry Suitable for transient and stable expression studies Features Strong CMV promoter for constitutive expression N-terminal DsRed fusion design Suitable for a broad range of mammalian cell types Sequence-verified construct