EF1α-EGFP Mammalian Expression Plasmid (pReg-EF1α-EGFP-v9)

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This vector is a mammalian expression plasmid designed for robust transgene expression in a wide range of mammalian cell types, including hard-to-transfect cell lines, stem cells, and primary cell cultures. It contains the enhanced green fluorescent protein (EGFP) driven by human elongation factor-1 alpha (EF1α) constitutive promoter for high, stable, long-term expression in mammalian cells. Plasmid contains ampicillin resistance for bacterial selection and neomycin resistance for stable selection with G418 in eukaryotic cells. Total vector size 6470 bp. Research Use Only (RUO). Applications Mammalian cell transfection EGFP fusion protein expression Fluorescence imaging Protein localization studies Live-cell microscopy Gene expression analysis Cell biology research Molecular cloning Features Strong EF1α promoter for high-level expression Enhanced Green Fluorescent Protein (EGFP) reporter Suitable for transient and stable expression studies Ideal for imaging and localization experiments Show more