/* ========================= Biofargo Product Page SOP ========================== */ .biofargo-wrap{ max-width:1000px; margin:0 auto; padding:10px; font-family:'Helvetica Neue', Helvetica, Arial, sans-serif; color:#333; line-height:1.6; } .biofargo-wrap .sec{ margin-bottom:30px; } .biofargo-wrap .sec h2{ padding-left:15px; color:#0056b3; font-size:20px; text-transform:uppercase; margin:0 0 15px 0; } .biofargo-wrap .desc-box{ background:#f9f9f9; padding:20px; border-radius:6px; border:1px solid #eee; } .biofargo-wrap .card{ border:1px solid #e1e1e1; padding:20px; border-radius:10px; background:#fff; } /* ========================= Table SOP ========================== */ .biofargo-wrap .biofargo-table-wrap{ overflow-x:auto; -webkit-overflow-scrolling:touch; border:1px solid #eee; border-radius:10px; padding:6px; background:#fff; margin:16px 0; } .biofargo-wrap table.biofargo-table{ width:100%; border-collapse:collapse; font-size:14px; background:#fff; } .biofargo-wrap table.biofargo-table th, .biofargo-wrap table.biofargo-table td{ border:1px solid #ddd; padding:12px; vertical-align:middle; word-break:break-word; white-space:normal; text-align:left; } .biofargo-wrap table.biofargo-table thead th{ background:#f2f2f2; font-weight:700; } .biofargo-wrap table.biofargo-table tbody td:first-child{ font-weight:700; background:#f8f9fa; } .biofargo-wrap table.w-640{ min-width:640px; } .biofargo-wrap ul{ padding-left:22px; margin:0; } .biofargo-wrap li{ margin:8px 0; } Description This product is a high-fidelity high-temperature resistant DNA polymerase isolated from the pyrophilus furiosis, with a molecular weight of 90 kDa, with 3'→5' exonuclease activity, no 5'→3' exonuclease activity, and its amplification product is blunt-end without protruding A. The elongation velocity of DNA polymerization was 600 bp/min, and the PCR product was blunt-end. Other high-temperature DNA polymerases, such as Vent, DeepVent, Tli, UlTma, etc., have correction functions, but Pfu has a very low error rate in amplifying DNA among all DNA polymerases that have been discovered so far. The following catalog numbers have been updated for improved product management:P310139-0100 → P310139-100UP310139-0200 → P310139-200UP310139-0500 → P310139-500U P310139-5000 → P310139-5KU Specification Parameter Specification Package 5 KU/100 U/200 U/500 U Full name Pfu DNA Polymerase Composition Pfu DNA polymerase (5 U/μL), 10X PCR Buffer (20 mM MgSO4). Reaction Buffer 10X PCR Buffer Unit Definition The amount of enzyme required to incorporate 10 nmol of dNTPs into acid-insoluble precipitates at 74°C for 30 min was defined as 1 activity unit (U). Concentration 5 U/μL Storage Buffer 20 mM Tris-HCl (pH 8.2); 0.1 mM EDTA; 0.1% Tween 20; 0.1% NP-40; 1 mM DTT; 100 mM KCl; 50% glycerol. Component Pfu DNA polymerase (5 U/μL), 10X PCR Buffer (20 mM MgSO4). Features The fidelity effect is 8 times higher than that of Taq enzyme. It is highly thermostable, and the viability of incubation at 95°C for 1 h still maintains more than 90%. Produces blunt-ended PCR products that bind modified nucleotides (e.g., biotin, digoxigenin, fluorescently labeled nucleotides). Applications Conventional PCR, high-fidelity PCR, site-directed mutagenesis, clonal expression. Storage -20°C