Thermo Scientific Pierce iCON Centrifugal Protein Concentrator – 7 mL Volume, 9K MWCO, Regenerated Cellulose Membrane, 25 Devices/Pack (MPN: 89884 / 89884A) The Thermo Scientific Pierce iCON 89884 (also cataloged under 89884A) is an instrument-grade, high-performance ultrafiltration centrifugal concentrator engineered for the rapid volumetric concentration, desalting, and buffer exchange of biological macromolecules. Designed to handle sample volumes up to 7 mL, each disposable device features a high-flux 9,000 Molecular Weight Cut-Off (9K MWCO) regenerated cellulose membrane. The unique, optimized conical geometry maximizes available membrane surface area while actively minimizing structural protein polarization and localized solute adsorption. This design enables fast processing cycles and an impressive sample recovery rate typically exceeding 90%. Compatible with standard swinging-bucket and fixed-angle laboratory centrifuge rotors, this single-step system safely achieves concentration factors greater than 150-fold in under 30 minutes. Technical Performance and Design Conical Geometry Optimization: Engineered with a specialized angled interior frame that funnels fluid along the high-surface-area membrane. This physical matrix reduces the polarization effect, preventing target proteins from accumulating or fouling the filter surface mid-run. 9K MWCO Regenerated Cellulose: Fitted with a premium, low-binding regenerated cellulose ultrafiltration membrane. Explicitly rated to trap and retain enzymes, antibodies, DNA plasmids, and biomolecules larger than 9,000 Daltons with absolute structural consistency. Built-In Safe Dead-Stop Pocket: Incorporates a reliable 10 µL dead-stop volume tracking reservoir at the base of the upper chamber. This safety pocket prevents spinning completely to dry states, safeguarding fragile proteins from structural precipitation, aggregation, and denaturation threats. Dual-Rotor Mechanical Balance: Structurally validated to balance perfectly within both high-throughput swinging-bucket rotors (preferred for perpendicular alignment, recommended at 2,500–4,500 × g) and rigid fixed-angle laboratory centrifuges (driven at 5,000–7,000 × g). Product Specifications Catalog Number / MPN: 89884 (Cross-referenced as 89884A or family line 87748) Manufacturer / Brand: Thermo Scientific / Pierce Biotechnology Maximum Sample Capacity: 7.0 mL (7 milliliters) working processing volume Molecular Weight Cut-Off (MWCO): 9,000 Daltons (9K MWCO) Membrane Substrate Type: Low-Adsorption High-Flux Regenerated Cellulose Dead-Stop Volume Limit: ~10 µL structural retention floor Chemical Compatibility Profile: Broadly resistant to standard biological buffers, neutral salts, dilute acids, and cell culture additives (Not compatible with strong organic solvents) Standard Pack Array: Single factory case containing 25 individual pre-assembled concentrator tubes and collection vials Key Benefits and Applications Protein Sample Bio-Concentration: The standard laboratory solution for rapidly increasing the concentration of low-abundance enzymes, monoclonal antibodies, and cell lysates prior to downstream analysis. Inline Diafiltration and Buffer Exchange: Allows researchers to efficiently strip out trailing salts, urea, or low-molecular-weight preservatives by running successive dilute-and-spin wash cycles, preparing samples for mass spectrometry or NMR sequencing. Downstream Assay Optimization: Maximizes sample signal intensity ahead of Western blotting, ELISA profiling, structural crystallization steps, and high-performance liquid chromatography injection runs. Scrape-Free Sample Recovery: The wide-mouth top housing allows standard 200 µL adjustable pipettes or elongated gel-loading
