Plasmodesmata: Methods and Protocols

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Plasmodesmata: Methods and Protocolsprovides a unique compendium of currently applied methods to explore plasmodesmata (PD). Opening with overviews of PD structure and their functi on during development as well as the role of viruses as models for addressing cellular mechanisms in macromolecular trafficking and movement through PD, the book continues with detailed experimental protocols for imaging PD, the isolation and structural analysis of PD, the analysis of PD conductivity and regulation, and for studying cellular mechanisms by which macromolecules are targeted to PD and transported through the channel. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Practical and easy to use,Plasmodesmata: Methods and Protocolsdelivers detailed information about specific practical procedures in order to enable their reproduction and application in PD-related research in laboratories around the world. Show more