Description Polyoxyethylene-80, also known as Tween 80 or Polysorbate 80 (CAS 9005-65-6), is a biotech-grade non-ionic surfactant widely used as an emulsifier, solubilizer, and stabilizer in biochemical, molecular biology, and cell culture research. This compound is chemically defined as polyoxyethylene (20) sorbitan monooleate, making it highly compatible with aqueous biological systems. Manufactured to biotech-grade standards, this Polyoxyethylene-80 is supplied as a yellow to amber viscous liquid with low impurity levels and excellent batch-to-batch consistency. It is commonly used in laboratory workflows to stabilize proteins and enzymes, improve reagent solubility, and reduce non-specific binding in sensitive biochemical and immunological assays. With a controlled hydroxyl value, low moisture content, and low acid value, this Tween 80 reagent is suitable for demanding applications in protein research, antibody-based assays, buffer formulation, and cell-free biochemical systems, where reproducibility and chemical stability are essential. Specifications Item Value Product Name Polyoxyethylene-80 (Tween 80 TM of ICI America) Grade Biotech Grade CAS No. 9005-65-6 Appearance Yellow to Amber Viscous Liquid Hydroxyl Value 65–82 mgKOH/g Moisture ≤2.0% Acid Value ≤2 mgKOH/g Saponification Value 43–55 mgKOH/g Storage 18–25℃ Features Biotech-grade Polyoxyethylene-80 (Tween 80 / Polysorbate 80) for laboratory research Non-ionic surfactant with excellent emulsifying, solubilizing, and stabilizing performance Low moisture content (≤2.0%) to support long-term chemical and biological stability Controlled hydroxyl, acid, and saponification values for consistent experimental results Supplied as a ready-to-use viscous liquid suitable for direct laboratory application Ideal for protein, enzyme, and biochemical research workflows Consistent quality supported by COA and SDS documentation Applications Polyoxyethylene-80 (Tween 80, Polysorbate 80) is widely used as a biochemical reagent and laboratory surfactant in the following applications: Protein and enzyme stabilization in buffer systems and formulation studies Reduction of non-specific binding in immunoassays such as ELISA and related techniques Component of washing buffers for biochemical and molecular biology assays Emulsification and solubilization of hydrophobic compounds in aqueous solutions Reagent formulation for cell-free and biochemical research systems General laboratory research and development applications requiring a reliable non-ionic surfactant Documents COA SDS Disclaimer: For laboratory research use only.
