Aquascape Pond Starter Bacteria helps to quickly establish the biological ecosystem for new ponds and spring start-ups, helping to support the nitrogen cycle and provide clean and healthy water conditions. Starting your season right with Pond Starter Bacteria will allow you to spend more time enjoying your water feature and less time maintaining it throughout the year. Pond Starter Bacteria is completely safe for fish, plants, pets, and wildlife. Can be used in water temperatures of 40° and above. More Information: Quickly establishes the biological ecosystem in ponds Great for new ponds and spring start-ups Safe for fish, plants, pets, and wildlife Easy-to-use pump top Dosing Instructions: Package Size 8oz / 236ml 16oz / 473ml 32oz / 946ml 1 gal / 3.78ltr 1 Pump Treats 100 gal / 378 L 100 gal / 378 L 100 gal / 378 L 600 gal / 2,268 L 1 Bottle Treats 5,000 gal / 18,900L 10,000 gal / 37,800L 20,000 gal / 75,600L 80,000 gal / 302,400L Packaged Weight 0.75 lb 1.25 lb 2.5 lb 8 lb CALCULATE POND SIZE: Length (feet) x Width (feet) x Average Depth (feet) x 7.48 = US Gallons HOW OFTEN: Treat once a week for two weeks. HOW TO: Add to the area with the most circulation. We recommend heavily aerating for at least 24 hours after application. PLEASE NOTE: Most effective in water temperatures above 40°F (4.5°C). Keep out of reach of children. Store at room temperature. Water Treatment Temperature Guide Water Treatment Pond Starter Bacteria Effects of Freezing Slight reduction, thaw & shake before use Storage Temperature (min-max) 50°F-104°F/ 10°C - 40°C Consequences of Storing Outside Range Product and package deterioration Usage Range (min-max water temperature) 40°F-100°F/ 4.4°C- 38°C Consequences of Using Outside Range Reduced effectiveness