Protein G IgG Binding Buffer Catalog number: B2016322 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 50 mL Molecular Weight or Concentration: N/A Supplied as: Solution Applications: a molecular tool for various biochemical applications Storage: 2-8°C Keywords: Protein G IgG Binding Buffer Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um. References Sensi S, Goebel A. Human Auto-IgG Purification from High Volume Serum Sample by Protein G Affinity Purification Bio Protoc. 2022 Dec 5;12(23):e4562. Pavan GL, Bresolin ITL, Muzio AFV, Cunha DC, Bueno SMA. Phospho-l-tyrosine-agarose chromatography: Adsorption of human IgG and its proteolytic fragments Biomed Chromatogr. 2019 Jan;33(1):e4397. de Sousa PLR, Tavares PAS, Teixeira EMTS, Dias NA, de A Lima M, Luna FMT, Gondim DR, de Azevedo DCS, Junior IJS. Experimental designs for optimizing the purification of immunoglobulin G by mixed-mode chromatography J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Sep 1;1125:121719. Fishman JB, Berg EA. Purification of Antibodies: Diethylaminoethyl (DEAE) Chromatography Cold Spring Harb Protoc. 2019 Jan 2;2019(1). Bottomley SP, Sutton BJ, Gore MG. Elution of human IgG from affinity columns containing immobilised variants of protein A J Immunol Methods. 1995 Jun 9;182(2):185-92. Reese HR, Shanahan CC, Lembo J, Tsonev L, Hirsh A, Menegatti S. Chromatographic assay to probe the binding energy and mechanisms of homologous proteins to surface-bound ligands J Chromatogr B Analyt Technol Biomed Life Sci. 2020 Jan 1;1136:121927. Ignat’eva NV, Ziganshina MM, Shilova NV, Khasbiullina NR, Bovin NV, Tyutyunnik VL, Sukhikh GT. Isolation of IgG Associated with Human Placenta Bull Exp Biol Med. 2019 May;167(1):120-122. Pinto ND, Uplekar SD, Moreira AR, Rao G, Frey DD. Immunoglobulin G elution in protein A chromatography employing the method of chromatofocusing for reducing the co-elution of impurities Biotechnol Bioeng. 2017 Jan;114(1):154-162. Strachan E, Mallia AK, Cox JM, Antharavally B, Desai S, Sykaluk L, O’Sullivan V, Bell PA. Solid-phase biotinylation of antibodies J Mol Recognit. 2004 May-Jun;17(3):268-76.
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