Description Proteinase K is a serine protease with wide cleavage activity, which can cleave the carboxyl-terminal peptide bond of aliphatic and aromatic amino acids. Its relative molecular weight is about 29.3 kDa. Proteinase K is widely used in the preparation of chromosomal DNA for pulse electrophoresis, Western blotting, and the removal of a nuclease from DNA and RNA preparation. Denaturant such as SDS (1%) can improve its activity. In addition, the common working concentration is 50-100 μg/mL, and the specific working concentration is according to whether the buffer contains SDS, urea, pH, temperature, and other factors. Applications Genomic DNA extraction Digestion and removal of the enzyme Specifications Source Yeast Specific Activity ≥30 U/mg Unit Definition One unit is defined as the amount of proteinase K that will liberate 1μmoL tyrosine per minute at 37℃ @ pH 7.5. Purification Ni column affinity purification Reaction Condition The reaction buffer should be incubated at 37℃ -70℃ for 1h, and the recommended reaction temperature is 70℃. Inactivation method The reaction system can be inactivated by adding PMSF or DFP inhibitor and partially inactivated by incubation at 65℃ for 10-15 min. Activity Range It has activity in a wide range of pH (4.0-12.0) and the optimal pH range is 7.5-9.0. RNase and DNase None Components Components No. Name 10401ES60 10401ES80 10401ES90 10401ES98 10401 Proteinase K 100 mg 1 g 5 g 100 g Shipping and Storage The product is shipped with ice packs and can be stored at 2℃ ~ 8℃ for three years. Documents: Safety Data Sheet 10401_MSDS_HB220429_EN_1652931562480.PDF Manuals 10401_Manual_HB221103_EN.pdf