ProTx-I Catalog number: MDP0772 CAS Number: 478507-53-8 Sequence: Glu-Cys-Arg-Tyr-Trp-Leu-Gly-Gly-Cys-Ser-Ala-Gly-Gln-Thr-Cys-Cys-Lys-His-Leu-Val-Cys-Ser-Arg-Arg-His-Gly-Trp-Cys-Val-Trp-Asp-Gly-Thr-Phe-Ser (Disulfide bonds between Cys2-Cys16, Cys9-Cys21, and Cys15-Cys28) Amount: 100 ug Molecular Weight: 3987.2 g/mol Supplied as: Powder Applications: molecular tool for various biochemical applications Storage: -20 °C WARNING: For in vitro Research Use Only. NOT for use on humans or animals. Grade: Biotechnology grade. All products are highly pure. References Priest BT, Blumenthal KM, Smith JJ, Warren VA, Smith MM. ProTx-I and ProTx-II: gating modifiers of voltage-gated sodium channels Toxicon. 2007 Feb;49(2):194-201. Rupasinghe DB, Herzig V, Vetter I, Dekan Z, Gilchrist J, Bosmans F, Alewood PF, Lewis RJ, King GF. Mutational analysis of ProTx-I and the novel venom peptide Pe1b provide insight into residues responsible for selective inhibition of the analgesic drug target Na(V)1.7 Biochem Pharmacol. 2020 Nov;181:114080. Ohkubo T, Yamazaki J, Kitamura K. Tarantula toxin ProTx-I differentiates between human T-type voltage-gated Ca2+ Channels Cav3.1 and Cav3.2 J Pharmacol Sci. 2010;112(4):452-8. Gui J, Liu B, Cao G, Lipchik AM, Perez M, Dekan Z, Mobli M, Daly NL, Alewood PF, Parker LL, King GF, Zhou Y, Jordt SE, Nitabach MN. A tarantula-venom peptide antagonizes the TRPA1 nociceptor ion channel by binding to the S1-S4 gating domain Curr Biol. 2014 Mar 3;24(5):473-83. Middleton RE, Warren VA, Kraus RL, Hwang JC, Liu CJ, Dai G, Brochu RM, Kohler MG, Gao YD, Garsky VM, Bogusky MJ, Mehl JT, Cohen CJ, Smith MM. Two tarantula peptides inhibit activation of multiple sodium channels Biochemistry. 2002 Dec 17;41(50):14734-47. Kikuchi K, Sugiura M, Kimura T. High Proteolytic Resistance of Spider-Derived Inhibitor Cystine Knots Int J Pept. 2015;2015:537508. Bladen C, Hamid J, Souza IA, Zamponi GW. Block of T-type calcium channels by protoxins I and II Mol Brain. 2014 May 9;7:36. Deplazes E, Henriques ST, Smith JJ, King GF, Craik DJ, Mark AE, Schroeder CI. Membrane-binding properties of gating modifier and pore-blocking toxins: Membrane interaction is not a prerequisite for modification of channel gating Biochim Biophys Acta. 2016 Apr;1858(4):872-82. Cardoso FC, Dekan Z, Smith JJ, Deuis JR, Vetter I, Herzig V, Alewood PF, King GF, Lewis RJ. Modulatory features of the novel spider toxin μ-TRTX-Df1a isolated from the venom of the spider Davus fasciatus Br J Pharmacol. 2017 Aug;174(15):2528-2544.
