Pseudomonas Isolation Agar Pseudomonas Isolation Agar is used for the selective isolation of Pseudomonas and differentiation of Pseudomonas aeruginosa based on pigment production. Enzymatic Digest of Gelatine provides nitrogenous sources for bacterial growth. The presence of Irgasan allows the growth of Pseudomonas spp. while suppressing that of Gram-positive and some Gram-negative microorganisms. Magnesium Chloride and Potassium Sulfate enhance the production of the iron chelators pyoverdin (yellow-green or yellow-brown fluorescent) and pyocianin (blue), whose combination produces a characteristic bright green color, by P. aeruginosa. Agar is the gelifying agent. There is a choice of 3 sizes - 500g, 2kg, and 10kg. PREPARATION: Mix 45 grams of the agar medium in 980 ml of purified water. Add 20 ml of glycerol while heating to facilitate drainage from pipet. Heat with repeated stirring until boiling to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes. Additional Literature: P16-114 Product Information
