Description Quantify both endogenous active MMP-8 and total MMP-8 The QuickZyme Human MMP-8 Activity Assay enables you to specifically measure in biological samples both active human MMP-8, as well as (pro)MMP-8 which is activated on the plate by APMA. It can be used for the measurement of MMP-8 activity in various biological samples, such as conditioned culture media, tissue homogenates, serum, plasma and urine.This 96-well plate format assay is based on the QuickZyme technology, using a modified pro-enzyme as a substrate, which upon activation is able to release color from a chromogenic peptide substrate. This multiplication step provides a unique assay sensitivity. Features Measures both endogenous active MMP-8 (naturally occurring) and total active MMP-8 (following activation with APMA). Samples: cell culture conditioned medium, serum, plasma, urine, saliva and tissue homogenates. Quantitative. Range: 10 – 24000 pg/ml. Sensitivity: 100 pg/ml for 2 h incubation with detection reagent; 24 pg/ml for 6 h incubation with detection reagent; 4 pg/ml for 24 h incubation with detection reagent. Ease-of-use: Equivalent to ELISA. Marker for neutrophils (PMN) Type Enzyme activity immunoassay Applications cell culture conditioned medium, serum, plasma, urine, saliva, tissue homogenates Sensitivity 100 pg/ml (2 hr incubation) 24 pg/ml (6 hr incubation) 4 pg/ml (24 hr incubation) Quantitative Measurement Typical data with 2 hours incubation. The shown data curve is provided for demonstration only. Storage Unopened kit: Store at -20°C, except for the standard, this vial should be stored at -70°C. Do not use kit, or individual kit components past kit expiration date. Opened kit / reconstituted reagents: Please refer to kit manual. Other names Matrix metalloproteinase-8, MMP8, Neutrophil collagenase, PMNL collagenase, PMNL-CL Resources Product Datasheet (PDS) Product Brochure Frequently Asked Questions (FAQ) Product References Lee, Jungwon et al. “Diagnostic Models for Screening of Periodontitis with Inflammatory Mediators and Microbial Profiles in Saliva.” Diagnostics (Basel, Switzerland) vol. 10,10 820. 14 Oct. 2020, doi:10.3390/diagnostics10100820 RELATED PRODUCTS Matrix Metalloproteinases Analysis Extracellular Matrix Assays Ilex Life Sciences LLC is an official distributor of QuickZyme Biosciences.
