Product Specifications: Item#1321: Rabbit Anti-gp120 MN HIV-1 Polyclonal Antibody (IgG) Concentration: See vial Mass/vial: See vial Diluent: PBS Purity: >95% Stabilizer: None Preservative: None Storage: -75°C Physical State: Frozen Liquid Stability: At least 12 months at -75°C Minimum Dilution: 1 : 1000 Application: Elisa/Western Elisa, Neutralization, Immunoprecipitation, Immunohistochemistry. Description: pAb IgG fraction purified from rabbit serum immunized with rgp120 HIV-1 MN Purification: This pAb IgG was purified by protein-A affinity chromatography to >95% purity as determined by SDS-PAGE, reduced. Specificity: This pAb IgG binds to native and recombinant HIV-1 gp120/gp160 in ELISA and Western ELISA. Biological Activity: This pAb IgG neutralizes HIV-1 as determined by syncycium assays using infected CD4 bearing cells and human PBL co-cultures. Application and Instructions for use: Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. This pAb IgG yields titres of greater than 1 : 2000 with 100ng of immobilized gp120 (ImmunoDx Product# 1001) under standard ELISA conditions. Western ELISA may be performed at 1-2µg/ml of pAb depending on the nature and quantity of the immobilized antigen. Histochemical studies require pAb in 1 : 200 dilution range. This antibody does not cross-react with other human or animal proteins.
