Product Specifications: Item#1302: Rabbit Anti-tat HIV-1 IIIB Polyclonal Antibody (IgG) Concentration: See vial Mass/vial: 100µg Diluent: PBS Purity: >90% Stabilizer: None Preservative: None Storage: -75°C Physical State: Frozen Liquid Stability: At least 12 months at -75°C Minimum Dilution: 1 : 1000 Application: Elisa/WesternElisa, Neutralization, Immunoprecipitation, Immunohistochemistry. Description: pAb IgG fraction purified from rabbit serum immunized with recombinant tat HIV-1 IIIB. Purification: This pAb IgG was purified by protein-A affinity chromatography to >95% purity as determined by SDS-PAGE, reduced. Specificity: This pAb IgG binds to native and recombinant HIV-1 IIIB tat in ELISA and Western ELISA. Biological Activity: Not determined. Application and Instructions for use: Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. This pAb IgG yields titres of greater than 1 : 2000 with 100ng of immobilized gp120 (ImmunoDiagnostics Product# 1001) under standard ELISA conditions. Western ELISA may be performed at 1-2µg/ml of pAb depending on the nature and quantity of the immobilized antigen. Histochemical studies require pAb in 1 : 200 dilution range. This antibody does not cross-react with other human or animal proteins.
