Paint with living color!A vibrant collection of six chromoprotein expression plasmids, inspired by corals and anemones, constitutively expressing in E. coli BL21 cells. These are kanamycin selected plasmids and only require to be streaked out and grown at 37C in LB agar or broth with 100mg/L Kanamycin to express. The proteins are extremely soluble and provide a wonderful teaching aid for all things microbiology. These plasmids are open source, made with love in Huntington, NY, and are free of any Material Transfer Agreements. The plasmids are licensed under Creative Commons 4.0 BY-SA.Plasmids in this collection:pIDMv5K-REDpIDMv5K-ORANGEpIDMv5K-YELLOWpIDMv5K-GREENpIDMv5K-BLUEpIDMv5K-PURPLE Please refer to my Plasmid Library for the sequence data of this plasmid.For a protocol on how to use this plasmid, please see my Protocols Library for step-by-step instructions.For the latest out of my research projects and hints at potential new products, check out my Open Lab Notebook, where all my work lives.The plasmid is shipped pre-transformed in a safe non-pathogenic laboratory line of E. coli in an agar stab. Please store at 4C in a fridge or at room temp in the dark. DO NOT FREEZE. Properly stored unopened agar stabs have lasted well over 5 years with good recovery in my lab. To maintain the plasmid in the E. coli it comes in, please use standard LB Agar with 100mg/L Kanamycin and incubate overnight at 37C. Please restreak when you would like to use the cells and only work with freshly grown well isolated colonies. Do not grow cells in liquid media directly as it will carry over stressed cells and lead to uneven colony sizes and poor growth performance. LIABILITY WAIVER By purchasing products from New York Botanics, LLC dba "Sebastian's Biotech Bazaar", henceforth referred to as the "Seller", you, the "Purchaser", agree to the following terms: 1. Acknowledgment of Risk: You acknowledge that the purchase and use of the plasmids involve known and unanticipated risks, which could result in physical or chemical damage, and you understand such potential risks. 2. Assumption of Risk: You knowingly, voluntarily, and expressly waive any claim you may have against the Seller for any such damages sustained as a result of purchasing or using the plasmids. 3. Release and Waiver: You hereby voluntarily release, forever discharge, and agree to indemnify and hold harmless the Seller from any and all claims, demands, or causes of action which are in any way connected with your purchase or use of the plasmids. 4. Severability: You expressly agree that the foregoing release and waiver, assumption of risk, and indemnity agreement are intended to be as broad and inclusive as permitted by law, and that if any portion thereof is held invalid, it is agreed that the balance shall continue in full legal force and effect. By purchasing this product, you acknowledge that you have read this release of liability and assumption of risk agreement, fully understand its terms, understand that you have given up substantial rights by agreeing to it, and agree to it freely and voluntarily without any inducement.
