Substrate Reagent Pack by R&D Systems · SKU DY999 Overview The Substrate Reagent Pack (Catalog # DY999) from R&D Systems is a set of chromogenic ELISA substrate solutions formulated to produce a blue color in the presence of horseradish peroxidase (HRP)-conjugated detection reagents and, upon acid stop, a yellow signal measurable at ~450 nm. The pack includes paired reagents that are mixed immediately prior to use to support color development in immunoassays and other HRP-based detection formats. This reagent pack is intended for research use in sandwich ELISAs and similar assays. Highlights Includes multiple vials of matched substrate reagents for ELISA colour development Optimised for use with HRP-conjugated detection systems Generates a measurable chromogenic signal at ~450 nm upon reaction and stopping Ready to mix and apply to microplate wells for consistent development At-a-glance Kit name Substrate Reagent Pack Contents 8 vials Colour Reagent A (stabilised hydrogen peroxide) and 8 vials Colour Reagent B (stabilised tetramethylbenzidine), ~12 mL/vial Application ELISA and HRP-based immunoassays Detection Chromogenic colour development ~450 nm Usage Reagents mixed 1:1 and added to wells for colour development Condition NEW — Unused reagent pack Applications This reagent pack is used in sandwich ELISAs and other immunoassays that employ HRP-conjugated detection antibodies or streptavidin-HRP to generate a colourimetric signal. Upon addition to assay wells and timed incubation, the TMB substrate system produces a quantifiable signal proportional to analyte concentration, suitable for research workflows in cytokine, growth factor, biomarker and protein quantification studies. Sustainability By rehoming surplus lab products, this listing supports waste reduction and resource efficiency.
