Defined Bioscience’s Ready-CEPT® 1000X cocktail is a defined formulation designed for improved stem cell survival and growth during routine passaging, cryopreservation, and freeze-thaw recovery. Ready-CEPT is a single-vial cocktail of chroman-1 (a ROCK inhibitor), emricasan (a pan-caspase inhibitor), polyamines (putrescine, spermine, and spermidine; polycations for cell growth support), and trans-ISRIB (an ISR inhibitor). CEPT was originally developed at the National Institutes of Health (NIH), where it was found to improve single-cell cloning and sorting, as well as improved viability and cell health. These benefits were well-characterized in particular for genetically stable stem cell culture, with benefits in cell passaging, cryoprotection, organoid and embryoid body (EB) formation, and gene editing, with fewer apparent off-target effects compared to other similar products on the market (e.g. Y-27632). Ready-CEPT is provided as a 1000X cocktail in a DMSO/water mixture, all using animal origin-free componentry. Ready-CEPT 1000X is provided in liquid form in 10- and 200-µL volumes, sufficient for 10 and 200 mL of CEPT-supplemented medium, respectively. This supplement can be used with a wide range of formulations, including Defined Bioscience’s fully defined HiDef-B8 medium (Catalog # LSS-204). Each lot of Ready-CEPT is used in combination with HiDef-B8 medium in performance testing in a culture assay evaluating using human iPSCs.
