Recombinant Betacellulin, Mouse Catalogue Numbers: -10, -50 Sizes: 10μg, 50μg Source: Escherichia coli Molecular Weight: 9.2 kDa, observed by reducing SDS-PAGE. Purity: > 95% by SDS-PAGE analysis. Biological Activity: ED50 2× 10ˆ6 units/mg. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. Formulation: Lyophilized after extensive dialysis against 50mM Tris, 300mM NaCl, pH9.0. AA Sequence: MDGNTTRTP ETNGSLCGAP GENCTGTTPR QKVKTHFSRC PKQYKHYCIH GRCRFVVDEQ TPSCICEKGY FGARCERVDL FY Endotoxin: < 0.2 EU/μg, determined by LAL method. Reconstitution: Reconstituted in ddH2O at 100 μg/mL. Storage: Lyophilized recombinant mouse Betacellulin (rmBetacellulin) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmBetacellulin remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description: Betacellulin is a pleiotropic cytokine that belongs to the Epidermal Growth Factor (EGF) family. Like other members of the EGF family, Betacellulin possesses a conserved sequence of 35-40 amino acids which contain 3 disulfide bonds formed by 6 cysteines. Betacellulin is unique in the EGF family since it can bind and activate a broad spectrum of ErbB receptors. Functionally, Betacellulin plays a role in the development of the pancreas by activating signaling pathways beneficial for the function, survival and regeneration of pancreatic β-cells. Additionally, Betacellulin has potential angiogenic activities and is important for the growth, development and repair of certain tissues.Recombinant mouse Betacellulin (rmBetacellulin) produced in E.coli is a single non-glycosylated polypeptide chain containing 81 amino acids. A fully biologically active molecule, rmBetacellulin has a molecular mass of 9.2 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
