.biofargo-table-wrapper {overflow-x: auto;margin: 20px 0;-webkit-overflow-scrolling: touch;}.biofargo-spec-table {width: 100%;border-collapse: collapse;border: 1px solid #dee2e6;min-width: 600px;background: #fff;}.biofargo-spec-table th,.biofargo-spec-table td {border: 1px solid #dee2e6;padding: 12px;vertical-align: middle;white-space: normal;word-break: break-word;}@media (max-width: 600px) {.biofargo-table-wrapper {border: 1px solid #eee;border-radius: 8px;padding: 6px;background: #fff;}.biofargo-spec-table {min-width: 720px;}} Description LN521E8 (Laminin-511 E8 Fragment) is an E8 fragment of laminin-521 that provides robust support for stem cell self-renewal and survival. It is commonly used for iPSC/ESC maintenance, single-cell passaging, and clinical-grade stem cell culture systems. Specifications Product Details Code Y13342HPurity≥95%, by SDS-PAGE (under reducing (R)& Non-reducing conditions, visualized by Coomassie staining). Endotoxin ≤10 EU/mg by the LAL method. Bioactivity Measured by the ability of the immobilized protein to support the adhesion of NIH3T3 cells. The ED50 for this effect is ≤10 ng/mL. Accession O15230&P55268&P11047 Species Human Expression System HEK293 Mol. Weight 141.0 KDa Expression Region Ala2534-Ala3322&Leu1573-Gln1798&Asn1364-Pro1609 Tag Info Tag free Formulation Lyophilized Buffer Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol, 0.01% Tween 80, pH7.4. Size 10μg/50μg/1mg Lead Time Products are in stock, but custom products may require further consultation. Reconstitution It is recommended to redissolve in sterile deionized water. Shipping The product is shipped with blue ice. Storage & Stability 36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Features ✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results. ✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells. ✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches. ✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements. Applications Hematology Supports HSC adhesion in vitro. on. Stem Cell Research Maintains ESC/iPSC pluripotency and improves survival in culture. Cell Biology Provides basement membrane cues, promotes adhesion and proliferati Data Figures Instructions for Use Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use. Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results. Do not mix with other similar products to avoid cross-reactivity and potential interference. Cautions • Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications. • Preservation: Handle according to instructions to preserve its activity and stability. • Support: Contact our technical support team if you encounter any issues or questions. Documents 📄 Download COA
