Recombinant PDGF-BB, Mouse Catalogue Numbers: BK0152-10, BK0152-50 Sizes: 10μg, 50μg Source: Escherichia coli Molecular Weight: 24.7 kDa, observed by non-reducing SDS-PAGE. Purity: > 95% by SDS-PAGE analysis. Biological Activity: ED50 < 2.5 ng/mL, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 4 × 10ˆ5 units/mg. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. Formulation: Lyophilized after extensive dialysis against 10 mM Sodium Citrate, pH 3.0. AA Sequence: MSLGSLAAA EPAVIAECKT RTEVFQISRN LIDRTNANFL VWPPCVEVQR CSGCCNNRNV QCRASQVQMR PVQVRKIEIV RKKPIFKKAT VTLEDHLACK CETIVTPRPV T Endotoxin: < 0.2 EU/μg, determined by LAL method. Reconstitution: Reconstituted in ddH2O at 100 μg/mL. Storage: Lyophilized recombinant mouse Platelet-Derived Growth Factor-BB (rmPDGF-BB) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmPDGF-BB remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description: Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, development of human skeleton, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.Recombinant mouse Platelet-Derived Growth Factor-BB (rmPDGF-BB) produced in E.coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 110 amino acids each. A fully biologically active molecule, rmPDGF-BB has a molecular mass of 24.7 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
