Recombinant Rat Fibroblast Growth Factor-basic (rRtbFGF) Catalogue Numbers: PR3002-10, PR3002-50, PR3002-1000 Sizes: 10µg, 50µg, 1.0mg (Contact us for the 1.0mg option) Source: Escherichia coli Molecular Weight:Approximately 16.3 kDa, a single non-glycosylated polypeptide chain containing 145 amino acids. Purity: >98% by SDS-PAGE and HPLC analyses. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of thymidine uptake by 3T3 cells is ≤ 0.2 ng/ml ,corresponding to a specific activity of ≥5 x 106 units/mg. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. Formulation: Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4. AA Sequence: PALPEDGGGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHV KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTEE CFFFERLESN NYNTYRSRKYSSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS Endotoxin: Less than 1EU/mg of rRabFGF as determined by LAL method. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at