PRODUCT SPECIFICATIONS ITEM# 5009: Recombinant SIV gp41 Envelope Protein (E.coli) SPECIFICATIONS Concentration: See vial Mass/vial: 0.1mg Volume/vial: see vial Diluent: 50 mM Tris, 0.1M NaCl, 2mM DDT, pH8 Purity: >98% Stabilizer: None Preservative: None Storage: -75oC Physical State: Frozen Liquid Stability: At least 24 months at -75 oC. DESCRIPTION: Truncated Recombinant SIV gp41 envelope protein produced in the E.coli Expression System. Truncated gp41 protein mol.wt: 28kD. Applications : In- Vitro Diagnostics,Immunization, Elisa/Western Blots. PURIFICATION: This protein was purified by solvent extraction and by preparative electrophoresis to >98% purity as determined by SDS-PAGE, reduced. SPECIFICITY: This protein binds to HIV-2 converted human serum polyclonal antibodies in ELISA and Western Elisa. BIOLOGICAL ACTIVITY: Not done APPLICATION AND INSTRUCTIONS FOR USE Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions.
