.biofargo-table-wrapper {overflow-x: auto;margin: 20px 0;-webkit-overflow-scrolling: touch;}.biofargo-spec-table {width: 100%;border-collapse: collapse;border: 1px solid #dee2e6;min-width: 600px;background: #fff;}.biofargo-spec-table th,.biofargo-spec-table td {border: 1px solid #dee2e6;padding: 12px;vertical-align: middle;white-space: normal;word-break: break-word;}@media (max-width: 600px) {.biofargo-table-wrapper {border: 1px solid #eee;border-radius: 8px;padding: 6px;background: #fff;}.biofargo-spec-table {min-width: 720px;}} Description FGF-2 (Fibroblast Growth Factor-2 (Basic)), also known as bFGF, is one of the earliest recognized members of the FGF family. Secreted by endothelial cells, smooth muscle cells, and macrophages, FGF-2 acts on a variety of cell types. As a crucial regulator of cell growth and differentiation, FGF-2 possesses biological activities such as pro-angiogenic, proliferative, chemotactic, and migratory effects, playing a vital role in processes of cell differentiation and organismal development. Specifications Product Details Cat. No. Y00321T Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). Endotoxin ≤200 EU/mg by the LAL method. Bioactivity Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast Cells. The ED50 for this effect ≤2.0 ng/mL. Accession XP_044218972.1 Alternative Names Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB Species Tuna Expression System E. coli Mol. Weight 17 kDa Expression Region Met1-Ser155 Tag Info Tag free Buffer Lyophilized from a 0.22 μm-filtered solution containing 10 mM PB, 5% Trehalose and 0.01% Tween 80, pH7.4. Size 50μg/1mg Lead Time Products are in stock, but custom products may require further consultation. Reconstitution It is recommended to redissolve in sterile deionized water. Shipping The product is shipped with blue ice. Storage & Stability 36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Features ✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results. ✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells. ✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches. ✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements. Applications Used for developing species-specific cell agriculture solutions and veterinary research applications. Data Figures Figure 1: Measured in a cell proliferation assay using Nb2-11 cells. Instructions for Use Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use. Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results. Do not mix with other similar products to avoid cross-reactivity and potential interference. Cautions • Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications. • Preservation: Handle according to instructions to preserve its activity and stability. • Support: Contact our technical support team if you encounter any issues or questions. Documents 📄 Download COA
