Product Specifications: Item# 2001: Recombinant HIV-2 (ROD) Glycoprotein gp105 Concentration: See vial Mass/vial: 100ug Volume/vial: See vial Diluent: PBS Purity: >90% Stabilizer: None Preservative: None Storage: -75°C Physical State: Frozen Liquid Stability: At least 12 months at -75°C. Application: Human Diagnostics,Drug Screening,CD4 Binding,Vaccines,Immunization,T-Cell Activation. Description: Full length Recombinant HIV-2 (ROD) gp105 glycoprotein produced in the Baculovirus Expression System. Purification: This protein is purified by immunoaffinity chromatography to >90% purity as determined by SDS-PAGE, reduced. Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and HIV-2 human serum polyclonal antibodies in ELISA and Western ELISA. Biological Activity: This protein binds to human T-cell receptor CD4, albeit with lower affinity compared to HIV-1 gp120, in ELISA and Western ELISA as determined by CD4/gp105/Anti- gp105 mAb-peroxidase capture ELISA. This protein activates human T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells. Application and Instructions for use Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 10-100ng of immobilized protein under standard ELISA conditions. Item#: 2001-P Peroxidase-Conjugated rgp105 HIV-2 (ROD) Application: Human diagnostics, CD4 binding, Drug screening, Human T-cell CD4 studies. Item#: 2001-G Colloidal gold-Conjugated rgp105 HIV-2 (ROD) Application: Human diagnostics, CD4 binding, Drug screening, FACS analysis. Item#: 2001-F Flourescene-Conjugated rgp105 HIV-2 (ROD) Application: FACS analysis, Histocytology. Item#: 2001-M Magnetically-Coupled rgp105 HIV-2 (ROD) Application: Human diagnostics, Human B and T-cell panning.
