Product Specifications: Item# 1011: Recombinant HIV-1 C Envelope Glycoprotein gp120 (Baculovirus) Concentration: See Vial Mass/vial: 100ug Diluent: PBS Purity: >95% Stabilizer: None Preservative: None Storage: -75oC Physical State: Frozen Liquid Stability: At least 2 years at -75oC. Applications: Vial In-Vitro Diagnostics, T-Cell Activation, CD4 Binding. Description: Full length Recombinant HIV-1 C gp120 glycoprotein produced in the Baculovirus Expression System. Purification: This protein is purified by immune-affinity chromatography to >95% purity as determined by SDS-PAGE, reduced. Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and human serum polyclonal antibodies in ELISA and Western ELISA. Biological Activity: This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp120/Anti-gp120 mAb-peroxidase capture ELISA. This protein activates human T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent Cells. Application and Instructions for use Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. HIV-converted human serum polyclonal antibodies yield titers of 1:1000 or greater at 1-10ng of immobilized protein under standard ELISA conditions.
