Product Specifications: Item# 5001: Recombinant SIVmac251 Glycoprotein gp130 (Baculovirus) Concentration: See vial Mass/vial: 100µg Volume/vial: see vial Diluent: PBS Purity: >95% Stabilizer: None Preservative: None Storage: -75°C Physical State: Frozen Liquid Stability: At least 12 months at -75°C. Application: Immunization, CD4 Binding, B & T-Cell Activation Description: Full length Recombinant SIV gp130 glycoprotein produced in the Baculovirus Expression System. Purification: This protein is purified by immunoaffinity chromatography to >90% purity as determined by SDS-PAGE, reduced. Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and simian serum polyclonal antibodies in ELISA and Western ELISA. Biological Activity: This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp130/Anti-gp130 mAb-peroxidase capture ELISA. This protein activates simian T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells. Application and Instructions for use Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 10-100ng of immobilized protein under standard ELISA conditions.
