Product Specifications: Item# 1004 : Recombinant HIV-1 IIIB Glycoprotein Soluble gp160 Concentration: See Vial Mass/Vial: 100µg Diluent: PBS In-Vitro Diagnostics Purity: >95% Stabilizer: None Preservative: None Storage: -75°C Physical State: Frozen Liquid Stability: 2 years at -75°C. Application: CD4 Binding, Vaccines, T- Cell Activation, In- Vitro Diagonostics. Description: Full length Recombinant HIV-1 IIIB soluble - membrane anchor sequence deleted- gp160 glycoprotein produced in the Baculovirus Expression System. Purification: This protein is purified by immunoaffinity Chromatography to >95% purity as determined by SDS-PAGE, reduced. Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificit and human serum polyclonal antibodies in ELISA and Western ELISA. Biological Activity: This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp160/Anti gp160 mAb-peroxidase capture ELISA. This protein activates human T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells. Applications and Instructions for use Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. HIV-1 converted human serum polyclonal antibodies yield titers of 1:1000 or greater at 1-10ng of immobilized protein under standard ELISA conditions. Item#: 1004-P Peroxidase-Conjugated rgp160 HIV-1 IIIB Application: Human diagnostics, CD4 binding, Drug screening, Human T-cell CD4 studies. Item#: 1004-G Colloidal gold-Conjugated rgp160 HIV-1 IIIB Application: Human diagnostics, CD4 binding, Drug screening, FACS analysis. Item#: 1004-F Flourescene-Conjugated rgp160 HIV-1 III Application: FACS analysis, Histocytology.
